Journal: Life

Article Title: Characterizing 3T3-L1 MBX Adipocyte Cell Differentiation Maintained with Fatty Acids as an In Vitro Model to Study the Effects of Obesity

doi: 10.3390/life13081712

Figure Lengend Snippet: Adipocyte secretions from differentiated 3T3-L1 MBX cells on Day 11 with and without the combination treatment of 3 kinds of fatty acids in DMEM after the initiation of differentiation from Day 6. ( A ) The dot blots indicate different concentrations of adipokines detected on nitrocellulose membrane after treatment with supernatants of (I) DMEM media without FBS, which has been used for conditioning cells (II) 3T3-L1 MBX cells from Day 11 treated with FBS-free DMEM in the absence of fatty acids cocktail (III) 3T3-L1 MBX cells from Day 11 treated with FBS-free DMEM in the presence of fatty acids cocktail. ( B ) Bar graphs of adipokines with the observable changes in adipokine secretions from differentiated 3T3-L1 MBX cells cultured in the presence or absence of fatty acids cocktail. Each data point represents the mean value of the secretome in 2 technical replicates for 3 independent experiments. ( C ) Fold change expression of secreted adipokines from differentiated 3T3-L1 MBX cells on Day 11 treated with fatty acids cocktail in respect to differentiated 3T3-L1 MBX cells on Day 11 treated without fatty acids. The statistical significance between the treatment group and the control was determined using a paired t -test. A p -value < 0.05 was considered statistically significant. One asterisk (*) indicates p ≤ 0.05, two asterisk (**) indicates p ≤ 0.01, and three asterisk (***) indicates p ≤ 0.001. p -value > 0.05 was considered statistically nonsignificant (ns).

Article Snippet: The adipocyte secretion profiling of the mature and obese 3T3-L1 MBX-derived media, 11 days after the initiation of differentiation, was perfomed using a proteome profiler mouse adipokine array kit (R & D System, Catalog# ARY013), following the manufacturer’s protocol.

Techniques: Membrane, Cell Culture, Expressing, Control

Journal: Life

Article Title: Characterizing 3T3-L1 MBX Adipocyte Cell Differentiation Maintained with Fatty Acids as an In Vitro Model to Study the Effects of Obesity

doi: 10.3390/life13081712

Figure Lengend Snippet: Calculated measure of difference in adipokine secretions from differentiated 3T3-L1 MBX cells cultured in the presence or absence of the fatty acids cocktail.

Article Snippet: The adipocyte secretion profiling of the mature and obese 3T3-L1 MBX-derived media, 11 days after the initiation of differentiation, was perfomed using a proteome profiler mouse adipokine array kit (R & D System, Catalog# ARY013), following the manufacturer’s protocol.

Techniques: Cell Culture

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Adipose stem cells exhibit mechanical memory and reduce fibrotic contracture in a rat elbow injury model

doi: 10.1096/fj.202001274R

Figure Lengend Snippet: Adipokine array results: Heatmap of normalized intensity ratio for Soft/Stiff and Soft-to-Stiff (1)/Stiff-to-Stiff (1) with statistical results from two-way analysis of variance (ANOVA) for stiffness and priming with post-hoc Bonferroni corrected with False Discovery Rate using the Benjamini-Hochberg method. * p < 0.05. ** p < 0.01.

Article Snippet: Growth media was collected after 72 hours of culture for detection of adipokines by Proteome Profiler (ARY016, R&D Systems, Minneapolis, MN, USA).

Techniques: